Cloning of Human Growth Hormone (hgh) in Ta Cloning Vector
نویسندگان
چکیده
The 22-KDa is the protein product of human growth hormone (hGH) gene isoform 1 of the pituitary hormone (Ahmed et. al., 1993). hGH gene was amplified with PCR from human growth hormone cDNA clone by specific designed primers containing extraterminals having Nco I and Bam HI restriction sites. The PCR product obtained was analyzed by enzyme digestion and DNA sequencing. The product was cloned in pCR II TOPO TA cloning vector to maintain its integrity. This was followed by transformation into TOP 10 competent E. coli. After transformation, we look for ligate hGH into Expression system to get the desired protein.
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